Engineering Bromodomains with a Photoactive Amino Acid by Engaging ‘Privileged’ tRNA Synthetases by Philip Mannes in Chemical Communications

February 19, 2020

From the article in Chemical Communications: Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting ‘interactome’. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by ‘privileged’ synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for ‘interactome’ profiling.